hmm let me see. today was quite an interesting day. yeah, quite a no. of things happened.
went to work, started off by doing imaging. yup, taking pics of cells and more cells. in 24 well plates. then i warmed up and change the medium for all my cells. 1 flask + the well plates one. while taking images there was suddenly a power outage, and the air con stopped working after that. started getting rather hot n stuffy in the cell culture room but then i was almost finishing. then i realise that cos normally the cell culture lab quite cold mah, but now no air con so the lens and other metal, cold parts started having water vapour condensing on them. couldnt see the image properly... had to wipe off all the water 1st. then just as i was finishing up (only lef the clearing up to do), got annoucement asking us to all evacuate nanos. decided to parafilm the medium n keep it in the fridge b4 going off. went back to main lab, take off lab coat, fan came rushing in to the lab asking everyone to leave, and check that no one left in the lab... so we all proceeded down... lol quite a while since we last evacuated the building liao. but happens quite often. i think the 3 or 4th during this current attachment liao. all false alarms. got once was cos the air in lvl 9 smell weird. they found later was due to some polluted air that got into the fresh air ducts at the top of the building... dunnoe how it happen/wat pollutant it was... but today... was cos the air con not working... and ppl complain v stuffy then they decided to evacuate everyone in case ppl start to faint... hmmm... air con went back on really soon after we came down tho. then i went to get the images and clean up, then went to look for fan... but then he was getting ready to go lunch with other lab officers... so joined them for lunch at ghim moh... haha good:D i more than 2week nvr go out for lunch liao (as in go holland v/drive/ghim moh, ok that day got treated at crystal jade but other than that v long nvr go out liao) haha finally see the char kway teow stall open... ok lah... waited abt 15 mins in line... food was ok lah... hmm... dunnoe. cant remember how nice one supposed to taste like liao.
showed kor the pics, no good... haiz. than later he came looking for me, quite happy... found some video abt the protocol, haha. put him in a good mood all day. was supposed to do assay for expt we did yest, but guess wat? some smart guy in my lab changed the speed of the orbital shaker from 25rpm to 200 rpm, and the caps came loss... dunnoe how much evaporated... in other words, we had no choice up to repeat... haiz. luckily he in a good mood.. else i dunnoe how loudly he'll be banging the table. and in part thks to the horrid ph adjusting time we had yest, things went much more smothly today. and he briefed me abt his project plans, smthing he hasnt done in ages. well in fact he hasnt talked properly to me in ages too... haiz. hmm thinking back, i think i know why he so moody these few days liao... but not sure if its also why he has been so moody the psat few weeks? oh wells. this guy is a big puzzle/mystery to figure out... lol. i said exactly the same thing abt hhx. yeah anyways that's how my day went... quite interesting maybe? oh wells. cya tmr...
went to work, started off by doing imaging. yup, taking pics of cells and more cells. in 24 well plates. then i warmed up and change the medium for all my cells. 1 flask + the well plates one. while taking images there was suddenly a power outage, and the air con stopped working after that. started getting rather hot n stuffy in the cell culture room but then i was almost finishing. then i realise that cos normally the cell culture lab quite cold mah, but now no air con so the lens and other metal, cold parts started having water vapour condensing on them. couldnt see the image properly... had to wipe off all the water 1st. then just as i was finishing up (only lef the clearing up to do), got annoucement asking us to all evacuate nanos. decided to parafilm the medium n keep it in the fridge b4 going off. went back to main lab, take off lab coat, fan came rushing in to the lab asking everyone to leave, and check that no one left in the lab... so we all proceeded down... lol quite a while since we last evacuated the building liao. but happens quite often. i think the 3 or 4th during this current attachment liao. all false alarms. got once was cos the air in lvl 9 smell weird. they found later was due to some polluted air that got into the fresh air ducts at the top of the building... dunnoe how it happen/wat pollutant it was... but today... was cos the air con not working... and ppl complain v stuffy then they decided to evacuate everyone in case ppl start to faint... hmmm... air con went back on really soon after we came down tho. then i went to get the images and clean up, then went to look for fan... but then he was getting ready to go lunch with other lab officers... so joined them for lunch at ghim moh... haha good:D i more than 2week nvr go out for lunch liao (as in go holland v/drive/ghim moh, ok that day got treated at crystal jade but other than that v long nvr go out liao) haha finally see the char kway teow stall open... ok lah... waited abt 15 mins in line... food was ok lah... hmm... dunnoe. cant remember how nice one supposed to taste like liao.
showed kor the pics, no good... haiz. than later he came looking for me, quite happy... found some video abt the protocol, haha. put him in a good mood all day. was supposed to do assay for expt we did yest, but guess wat? some smart guy in my lab changed the speed of the orbital shaker from 25rpm to 200 rpm, and the caps came loss... dunnoe how much evaporated... in other words, we had no choice up to repeat... haiz. luckily he in a good mood.. else i dunnoe how loudly he'll be banging the table. and in part thks to the horrid ph adjusting time we had yest, things went much more smothly today. and he briefed me abt his project plans, smthing he hasnt done in ages. well in fact he hasnt talked properly to me in ages too... haiz. hmm thinking back, i think i know why he so moody these few days liao... but not sure if its also why he has been so moody the psat few weeks? oh wells. this guy is a big puzzle/mystery to figure out... lol. i said exactly the same thing abt hhx. yeah anyways that's how my day went... quite interesting maybe? oh wells. cya tmr...
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